P58IPK通过PERK/eIF2α通路调控猪圆环病毒2型所诱导的细胞自噬

doi:10.11843/j.issn.0366-6964.2017.11.014

Abstract

Abstract:

This experiment was conducted to study the relationship between porcine circovirus type 2 (PCV2) infection activated PERK/eIF2α pathway and autophagy, and the possible function of overexpressing chaperone P58IPK within it. PK-15 cells were treated with PERK specific inhibitor GSK2606414, siRNA, followed by PCV2 infection for 24 h. Western blot was taken to measure phosphorylated eIF2α (p-eIF2α), LC3-Ⅱ and ATG5-ATG12 expression at first. Then P58IPK overexpression vector was constructed and transiently transfected, combined with Western blot to study the influence of overexpressing P58IPK on PCV2-induced PERK/eIF2α pathway and autophagy. Western blot results showed PERK specific inhibitor GSK2606414 and siRNA significantly suppressed PCV2-induced p-eIF2α (P<0.01) and LC3-Ⅱ (P<0.01) expression. While overexpressing P58IPK not only resulted in PCV2-induced p-eIF2α (P<0.01), ATG5-ATG12 (P<0.01) and LC3-Ⅱ (P<0.01) decrease, but also significantly downregulated activating PCV2 copies number (P<0.01). Results indicate PCV2 infection induces autophagy through activating PERK/eIF2α pathway. Overexpression of P58IPK represses PCV2-induced autophagy through inhibiting PERK pathway, consequently, inhibiting PCV2 replication.

CLC Number:

S852.659.2

YANG Liao-han, WANG Kai, XU Su-tong, ZHANG Min, HU Lin, HE Qi-gai, ZHANG Shu-jun. P58IPK Regulates Porcine Circovirus Type 2-induced Autophagy through PERK/eIF2α Pathway[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2125-2134.

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P58IPK Regulates Porcine Circovirus Type 2-induced Autophagy through PERK/eIF2α Pathway

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